ZNF28 Knockout cell line (Caco-2)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZNF28 (zinc finger protein 28) encodes a predicted zinc finger-containing transcription factor capable of sequence-specific DNA binding within RNA polymerase II regulatory regions. Through its predicted transcription factor activity, ZNF28 may regulate expression of genes transcribed by RNA polymerase II. The protein is predicted to function primarily in the nucleus, consistent with a role in transcriptional regulation. However, its specific target genes, molecular partners, and physiological functions remain incompletely characterized.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.