ZNF280A Knockout cell line (HK-2)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZNF280A (zinc finger protein 280A) encodes a zinc finger protein containing four C2H2-type zinc finger domains. These domains commonly mediate interactions with DNA or RNA and are characteristic of many transcriptional regulators. ZNF280A may therefore participate in nucleic acid-dependent regulation of gene expression, although its specific molecular targets, regulatory mechanisms, and biological functions remain incompletely characterized.

HK-2 (Human Kidney-2) is a well-characterized immortalized human kidney proximal tubular epithelial cell line derived from normal adult renal tissue. Transformed via the transduction of human papillomavirus type 16 (HPV-16) E6/E7 genes, HK-2 successfully achieves continuous in vitro proliferation while stably retaining the functional and phenotypic characteristics of primary proximal tubular epithelium. HK-2 cells maintain distinct brush border enzyme activities and exhibit physiologic responsiveness to cytokines and nephrotoxic compounds. Consequently, HK-2 serves as a globally accepted, standard human cell platform extensively utilized in in vitro nephrotoxicity screening, renal fibrosis modeling, tubular transport mechanism profiling, and preclinical drug safety evaluations.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.