ZNF318 Knockout cell line (Hep G2)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZNF318 (zinc finger protein 318) encodes a predicted zinc finger-containing protein with protein homodimerization and heterodimerization activities. It is predicted to participate in both positive and negative regulation of DNA-templated transcription and is localized to the cytosol and nucleoplasm. These activities suggest a role in transcriptional regulation through protein-protein interactions, although the specific molecular mechanisms remain incompletely characterized.

A human liver tumor cell line established from the tumor tissue of a 15-year-old Caucasian male in 1975, originally described as a well-differentiated hepatocellular carcinoma and later reclassified by some sources as a hepatoblastoma. The cells produce multiple plasma proteins, including albumin, alpha-fetoprotein, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, and plasminogen. Hepatitis B virus surface antigens have not been detected, and the cells respond to stimulation with human growth hormone.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.