ZNF354B Knockout cell line (SU-DHL-1)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZNF354B (zinc finger protein 354B) encodes a zinc finger protein predicted to function as a sequence-specific transcription factor and transcriptional corepressor. It is predicted to bind cis-regulatory DNA regions associated with RNA polymerase II-dependent transcription and to negatively regulate transcription. The protein is predicted to function in the nucleus, where it may contribute to control of gene expression through DNA binding and transcriptional repression.

The SU-DHL-1 cell line was established in 1973 from the pleural effusion of a 10-year-old male patient. Originally diagnosed as diffuse histiocytic lymphoma, the tumor was subsequently reclassified as diffuse large cell lymphoma and later as malignant histiocytosis. According to the REAL/WHO classification, the cell line represents anaplastic large cell lymphoma (ALCL) and carries the t(2;5)(p23;q35) translocation resulting in an NPM1-ALK (NPM-ALK) fusion gene. SU-DHL-1 is used as a model for studying ALCL biology and ALK-associated oncogenic signaling.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.