ZNF41 Knockout cell line (HEK293)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZNF41 (zinc finger protein 41) encodes a Kruppel family zinc finger protein containing KRAB-A and KRAB-B domains together with multiple zinc finger DNA-binding motifs. These structural features are characteristic of transcriptional regulators involved in DNA binding and gene expression control. The specific biological functions and transcriptional targets of ZNF41 remain incompletely characterized.

The HEK293 cell line is a human embryonic kidney cell line derived from adenovirus 5-transformed human embryonic kidney cells. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions. HEK293 cells have been adapted into various derivatives, including suspension-adapted variants and engineered reporter lines for specific applications. This cell line is widely used in molecular biology, viral vector production, recombinant protein expression, gene expression studies, and research involving cellular signaling and genome engineering.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.