ZNF496 Knockout cell line (A549)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZNF496 (zinc finger protein 496) encodes a zinc finger DNA-binding transcription factor predicted to recognize RNA polymerase II cis-regulatory regions. It is involved in the positive regulation of DNA-templated transcription and broader regulation of RNA polymerase II-dependent gene expression. The protein is predicted to function in the nucleus, where its sequence-specific DNA-binding activity may contribute to transcriptional control and regulation of cellular gene expression.

A549 is a human lung cancer cell line established in 1972 from an explanted lung tumor of a 58-year-old male patient. The cells exhibit characteristics of alveolar type II pulmonary epithelial cells, including the ability to synthesize lecithin through the cytidine diphosphocholine pathway. A549 cells have been described as originating from lung adenocarcinoma and are widely used as a model for studying lung cancer biology, pulmonary epithelial function, drug responses, and respiratory-related research.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.