ZNF502 Knockout cell line (HEK293)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZNF502 (zinc finger protein 502) encodes a predicted zinc finger transcription factor with sequence-specific DNA-binding activity. ZNF502 is involved in regulation of transcription by RNA polymerase II and is localized to the nucleus. It has also been implicated in host regulation of viral processes and viral release, suggesting a potential role in cellular responses to viral infection, although the molecular mechanisms remain incompletely characterized.

The HEK293 cell line is a human embryonic kidney cell line derived from adenovirus 5-transformed human embryonic kidney cells. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions. HEK293 cells have been adapted into various derivatives, including suspension-adapted variants and engineered reporter lines for specific applications. This cell line is widely used in molecular biology, viral vector production, recombinant protein expression, gene expression studies, and research involving cellular signaling and genome engineering.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.