ZNF584 Knockout cell line (HCT-15)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZNF584 (zinc finger protein 584) encodes a predicted zinc finger transcription factor that may bind specific cis-regulatory regions associated with RNA polymerase II-dependent transcription. Through sequence-specific DNA binding, ZNF584 is predicted to participate in regulation of gene expression in the nucleus. The available annotation does not establish specific target genes, cellular pathways, or physiological functions, and its molecular mechanisms remain incompletely characterized.

HCT-15 is a human colorectal adenocarcinoma cell line established from a colorectal tumor. The cells are tumorigenic in nude mice and share a common genetic origin with several colorectal cancer cell lines, including DLD-1, HCT-8, and HRT-18, as confirmed by STR profiling. HCT-15 is widely used as a model for studying colorectal cancer biology, tumorigenesis, genetic alterations, and anticancer drug responses.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.