ZNF627 Knockout cell line (THP-1)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZNF627 (zinc finger protein 627) encodes a zinc finger protein predicted to possess DNA-binding and RNA polymerase II-specific transcription factor activity. It is predicted to bind transcriptional regulatory regions and participate in regulation of RNA polymerase II-dependent gene expression. Its predicted nuclear localization is consistent with a role in transcriptional regulation, although its specific biological functions remain incompletely characterized.

THP-1 is a human monocytic leukemia cell line established from the peripheral blood of a 1-year-old male patient with acute monocytic leukemia at relapse. The cells exhibit monocytic characteristics, including expression of Fc and complement receptors, lysozyme production, esterase activity, and phagocytic capacity. THP-1 cells can be differentiated into macrophage-like cells under appropriate stimulation and are widely used as a model for studying monocyte/macrophage biology, immune responses, inflammation, differentiation, and host–pathogen interactions.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.