ZNF703 Knockout cell line (Ishikawa)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZNF703 (zinc finger protein 703) encodes a zinc finger-containing regulatory protein involved in transcriptional and signaling processes. ZNF703 participates in regulation of mammary gland epithelial cell proliferation and transforming growth factor-β receptor signaling and may contribute to cellular responses to estradiol. Its localization to the cytoplasm and nuclear matrix suggests functions extending beyond direct DNA binding. Through these activities, ZNF703 may influence epithelial cell growth and signaling, although its precise molecular mechanisms remain incompletely characterized.

The Ishikawa cell line was established from an endometrial adenocarcinoma of a 39-year-old female patient. This human endometrial cancer model exhibits tumorigenic properties, forming well-differentiated adenocarcinomas in athymic nude mice. Ishikawa cells express estrogen and progesterone receptors both in vitro and in induced tumors, and they respond to steroid hormone stimulation. The cells have also been reported to produce corticotropin-releasing hormone, placental alkaline phosphatase, and chorionic gonadotropin. Ishikawa is widely used as a model for studying endometrial cancer biology, hormone receptor signaling, reproductive endocrinology, and endocrine-based therapeutic research.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.