ZNF785 Knockout cell line (THP-1)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZNF785 (zinc finger protein 785) encodes a predicted zinc finger transcription factor that may bind RNA polymerase II transcriptional regulatory regions in a sequence-specific manner. It is predicted to participate in regulation of RNA polymerase II-dependent transcription and to function in the nucleus. The specific transcriptional targets and biological functions of ZNF785 remain incompletely characterized.

THP-1 is a human monocytic leukemia cell line established from the peripheral blood of a 1-year-old male patient with acute monocytic leukemia at relapse. The cells exhibit monocytic characteristics, including expression of Fc and complement receptors, lysozyme production, esterase activity, and phagocytic capacity. THP-1 cells can be differentiated into macrophage-like cells under appropriate stimulation and are widely used as a model for studying monocyte/macrophage biology, immune responses, inflammation, differentiation, and host–pathogen interactions.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.