ZNF789 Knockout cell line (Caco-2)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

ZNF789 (zinc finger protein 789) encodes a zinc finger protein predicted to function as a sequence-specific DNA-binding transcription factor for RNA polymerase II-regulated transcription. It is predicted to bind cis-regulatory regions and participate in regulation of RNA polymerase II-dependent gene expression. ZNF789 is localized to the nucleoplasm, although its specific target genes and biological functions remain incompletely characterized.

Caco-2 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 72-year-old male patient. The cells can form differentiated intestinal epithelial-like monolayers with characteristics including microvilli, tight junctions, dome formation, and transport functionality. Caco-2 cells also produce mucus and can form moderately differentiated adenocarcinoma-like tumors in nude mice. The cell line is widely used as an in vitro model for studying intestinal epithelial differentiation, barrier function, permeability, drug absorption, and colorectal cancer biology.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.