ZNRF1 Knockout cell line (HCT-15)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZNRF1 (zinc and ring finger 1) encodes an E3 ubiquitin-protein ligase containing zinc finger and RING finger motifs. The protein localizes to endosomal and lysosomal compartments and is also associated with synaptic vesicle membranes in neurons. ZNRF1 participates in ubiquitin-mediated protein modification and has a role in neural cell differentiation. Overexpression of ZNRF1 can promote neurite-like cellular elongation, supporting a role in neuronal morphology.

HCT-15 is a human colorectal adenocarcinoma cell line established from a colorectal tumor. The cells are tumorigenic in nude mice and share a common genetic origin with several colorectal cancer cell lines, including DLD-1, HCT-8, and HRT-18, as confirmed by STR profiling. HCT-15 is widely used as a model for studying colorectal cancer biology, tumorigenesis, genetic alterations, and anticancer drug responses.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.