ZSCAN25 Knockout cell line (Hep G2)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZSCAN25 (zinc finger and SCAN domain containing 25) encodes a protein containing domains characteristic of zinc finger proteins, which commonly participate in DNA binding and protein-protein interactions. ZSCAN25 is therefore predicted to have roles in nuclear regulatory processes, although its specific molecular function and biological targets remain incompletely characterized. Available transcript information does not establish a clearly defined functional role for the protein.

A human liver tumor cell line established from the tumor tissue of a 15-year-old Caucasian male in 1975, originally described as a well-differentiated hepatocellular carcinoma and later reclassified by some sources as a hepatoblastoma. The cells produce multiple plasma proteins, including albumin, alpha-fetoprotein, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, and plasminogen. Hepatitis B virus surface antigens have not been detected, and the cells respond to stimulation with human growth hormone.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.