ZUP1 Knockout cell line (MDA-MB-231)

Ubigene gene knockout cell lines mediated by optimized CRISPR-Cas9 technology provide reliable in vitro models for investigating gene function, signaling pathways, disease mechanisms, and therapeutic targets.

ZUP1 (zinc finger containing ubiquitin peptidase 1) encodes a zinc finger-containing cysteine peptidase that functions as a K63-specific deubiquitinating enzyme. ZUP1 cleaves K63-linked polyubiquitin chains within the chain rather than removing terminal ubiquitin molecules. This activity contributes to regulation of K63-linked ubiquitin signaling and is implicated in DNA repair by processing ubiquitin chains at DNA repair sites.

MDA-MB-231 is a human breast cancer cell line established from the pleural effusion of a 51-year-old female patient with metastatic invasive breast carcinoma after chemotherapy. The cells are triple-negative breast cancer (TNBC) cells and are tumorigenic in nude mice. MDA-MB-231 is a widely characterized model used for studying breast cancer progression, metastasis, tumor biology, molecular signaling, and therapeutic responses.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.