ZWINT Knockout cell line (MDA-MB-231)

Gene knockout cell lines are generated using CRISPR-Cas9 based technology to precisely disrupt target genes, enabling functional genomics research, disease modeling, and target validation.

ZWINT (ZW10 interacting kinetochore protein) encodes a kinetochore-associated protein involved in chromosome segregation during mitosis. ZWINT interacts with ZW10 and is recruited to kinetochores during prophase before ZW10, remaining detectable through late anaphase. Its localization and interactions suggest a role in kinetochore assembly or regulation and in proper chromosome movement during cell division. The precise molecular mechanisms by which ZWINT supports kinetochore function remain incompletely characterized.

MDA-MB-231 is a human breast cancer cell line established from the pleural effusion of a 51-year-old female patient with metastatic invasive breast carcinoma after chemotherapy. The cells are triple-negative breast cancer (TNBC) cells and are tumorigenic in nude mice. MDA-MB-231 is a widely characterized model used for studying breast cancer progression, metastasis, tumor biology, molecular signaling, and therapeutic responses.

Before delivery, each clone is authenticated by STR, tested for sterility and mycoplasma, and genotype‑verified by two‑round PCR‑sequencing. Comprehensive QC data accompany every shipment to support reproducible research.