Zc3h3 Knockout cell line (RAW 264.7) – Genotype Verified & Ready-to-use

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Zc3h3 Knockout cell line (RAW 264.7)

Copy Product Info

Catalog#

YKO-MB22314

Species

Mouse

Target Cell Line

RAW 264.7

Size

1*10^6

Gene

Zc3h3

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Overview

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

Zdhhc12 (zinc finger, DHHC domain containing 12) encodes a protein with protein-cysteine S-palmitoyltransferase activity. It catalyzes the addition of palmitate to cysteine residues in proteins, a modification that can influence protein localization and function. ZDHHC12 participates in gephyrin clustering during postsynaptic density assembly and positively regulates GABAergic synaptic transmission. It is located in the Golgi apparatus and is expressed in the early conceptus, inner cell mass, and oocyte. Its human ortholog is ZDHHC12.

The RAW 264.7 cell line was established from the ascites of a tumor induced in a male mouse by intraperitoneal injection of Abelson murine leukemia virus (A-MuLV). This murine macrophage-like cell line exhibits functional characteristics associated with macrophages, including pinocytosis of neutral red, phagocytosis of zymosan particles, and antibody-dependent lysis of target cells. RAW 264.7 cells are responsive to lipopolysaccharide (LPS), which inhibits cell growth, and are widely used as an in vitro model for studying macrophage activation, innate immune responses, inflammatory signaling, and related immunological processes.

Comprehensive quality control includes STR authentication, sterility testing (bacteria and fungi), and mycoplasma screening to ensure purity and performance. Genotype is confirmed by two rounds of PCR and Sanger sequencing, and complete validation reports are provided for make-to-order products.

KO Cell Construction Strategy

Adopting Red Cotton CRISPR Gene Editing Designer (www.rc-crispr.com), two sgRNAs targeting the Zc3h3 gene are designed to generate a knockout strategy. The two sgRNA sequences are subsequently cloned into the EZ-editor™ vector and introduced into RAW 264.7 cells via electroporation or lentiviral transduction. Single-cell clones are then generated using the limiting dilution method. Genomic DNA from individual clones is subjected to nucleic acid lysis and PCR amplification using the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000). The edited loci are further verified by Sanger sequencing to confirm the genotype. After secondary validation and quality confirmation, Zc3h3 Knockout cell line (RAW 264.7) is expanded and cryopreserved for downstream applications.

Product Overview

Zc3h3 Knockout cell line (RAW 264.7)

The Zc3h3 Knockout cell line (RAW 264.7) is generated by targeted disruption of the Zc3h3 gene in RAW 264.7 cells. This cell line is designed to investigate the role of Zc3h3 in cellular function and gene regulation, as well as its potential involvement in disease-related pathways.

KO Cell Line

Zc3h3 Knockout cell line (RAW 264.7)

Gene ID

223642

Catalog

YKO-MB22314

Gene Name

Zc3h3

Product Category

Knockout(KO) Cell Line

Gene Information

NCBI

Species

Mouse

Mycoplasma test

Negative

Cell Morphology

monocyte/macrophage,adherent

Catalog Status

-

Culture method

90%DMEM+10%FBS

Shipping

Cryopreserved Cells shipped in dry ice

Passage ratio

1:2-1:3

Storage Condition

Liquid nitrogen

Quality Control

All KO cell products are tested and confirmed to be free of bacterial, viral, mycoplasma contamination, and other adventitious agents.

Applications

For research use only. Not intended for use in diagnostic, therapeutic, clinical, or other applications.

Key Advantages

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Quality Control Specifications

1. Bacteria and Fungi Test

None Detected.

2. Mycoplasma test

Negative.

3. STR Authentication

Download STR Report

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.

Cell Reception

2.

Unpacking and storage instructions

Handling procedure

1.

Cell Thawing

2.

Cell Passaging

3.

Cell cryopreservation

References

More

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?

If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:

(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.

(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.

Q2. What Information Is Required When Reporting KO Cell Product Issues?

Q3. How Should the KO cell Product Be Stored Upon Receipt?

Q4. Shipping Method

Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?