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Human Colon Carcinoma Cell line (HT-29)

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Human Colon Carcinoma Cell line (HT-29)
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Catalog#
YC-C008
Size
1*10^6 Cells/vial (frozen vial)
Culture method
90%McCOY's 5A+10% FBS
Instruction
STR Report

Overview

The HT-29 line was isolated from a primary tumor in 1964 by J. Fogh using the explant culture method.

Detailed Product Information

Human Colon Carcinoma Cell line (HT-29)
This cell line has a well-defined origin, a clean background, and broad applicability. It can be reliably used in basic research applications such as functional validation, signaling pathway analysis, and drug treatment.
Product name
Human Colon Carcinoma Cell line (HT-29)
Mycoplasma test
Negative
Catalog number
YC-C008
Cellosaurus ID
CVCL_0320
Product category
Wild-type Cell Lines
Shipping condition
Frozen vials; dry-ice package
Cell morphology
Epithelial-like, adherent
Storage condition
Liquid nitrogen
Culture Medium
90%McCOY's 5A+10% FBS
Species/Source
Human Colon Carcinoma Cell line
Passage ratio
1:2~1:4
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Sterility Testing
All cells have been tested and are free of bacteria, viruses, mycoplasma, and other toxins.

Recommended transfection method

Electroporation
Lentivirus

Quality Control Specifications

1. Bacteria and fungi test
The test result was negative.
2. Mycoplasma test
The test result was negative.
3. STR Authentication

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

References

FAQ

Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?
If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:
(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.
(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.
Q2. What Information Is Required When Reporting KO Cell Product Issues?
Q3. How Should the KO cell Product Be Stored Upon Receipt?
Q4. Shipping Method
Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?

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