Human Breast Adenocarcinoma Cell Line (SK-BR-3)
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Wild-type Cell Lines
Human Breast Adenocarcinoma Cell Line (SK-BR-3)
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Overview
This cell line was derived by G. Trempe and L. J. Old in 1970 from pleural effusion cells of a patient, a White, Caucasian female, age 43, blood type A+, who had been treated with radiation, steroids, cytoxan and 5-fluorouracil.
Detailed Product Information
Human Breast Adenocarcinoma Cell Line (SK-BR-3)
This cell line has a well-defined origin, a clean background, and broad applicability. It can be reliably used in basic research applications such as functional validation, signaling pathway analysis, and drug treatment.
Product name
Human Breast Adenocarcinoma Cell Line (SK-BR-3)
Mycoplasma test
Negative
Catalog number
YC-C048
Cellosaurus ID
CVCL_0033
Product category
Wild-type Cell Lines
Shipping condition
Frozen vials; dry-ice package
Cell morphology
Epithelial-like, adherent
Storage condition
Liquid nitrogen
Culture Medium
90%McCOY's 5A+10%FBS
Species/Source
Human Breast Adenocarcinoma Cell Line
Passage ratio
1:2~1:3
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Sterility Testing
All cells have been tested and are free of bacteria, viruses, mycoplasma, and other toxins.
Recommended transfection method
Quality Control Specifications
1. Bacteria and fungi test
The test result was negative.
2. Mycoplasma test
The test result was negative.
3. STR Authentication
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
Unpacking and Storage Instructions
1.
Cell Reception
2.
Unpacking and storage instructions
Handling procedure
1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation
References
FAQ
Q1. How to Contact Support if Issues Arise After Receiving the KO Cells?
If any abnormalities are observed after receiving the cells, please provide the following information based on the stage of use and contact us promptly for assistance:
(1) Issues upon delivery: Please provide photos of the cells at the time of receipt along with a brief description of the issue. We will offer after-sales support accordingly.
(2) Issues after thawing or passaging: Please provide microscopic images of the cells before and after thawing/passaging, along with a detailed description of the experimental procedures. Our technical team will provide professional support.
Q2. What Information Is Required When Reporting KO Cell Product Issues?
Q3. How Should the KO cell Product Be Stored Upon Receipt?
Q4. Shipping Method
Q5. Are the Knockout Cell Lines Stable for Passaging? Is Authentication Provided?
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