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ABCC3 Stable Expression Cell Line (MDCK)

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ABCC3 Stable Expression Cell Line (MDCK)
Size
1*10^6
Culture method
89%MEM+10%FBS+1% NEAA
Instruction
Detailed Product Information
ABCC3 Stable Expression Cell Line (MDCK)

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Product category
Stable Cell Lines
Transcript
NM_001359638.1
Cell Name
ABCC3 Stable Expression Cell Line (MDCK)
Species
Human
Gene Name
ABCC3
Passage ratio
1:3~1:4
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=3μg/ml
Culture method
89%MEM+10%FBS+1% NEAA
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamehABCC3-1CтMeandog-1CтMeanΔCтMeanΔΔCтExpression Rate
ABCC3 Stable Expression Cell Line (MDCK)17.71719.896-2.179-10.8301820.350
MDCK_Ctrl(Puro)28.31719.6668.6510.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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