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CD9 Stable Expression Cell Line (LN-229)

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CD9 Stable Expression Cell Line (LN-229)
Size
1*10^6
Culture method
90%DMEM+10%FBS
Instruction
STR Report
Detailed Product Information
CD9 Stable Expression Cell Line (LN-229)

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

Product category
Stable Cell Lines
Transcript
NM_001413241.1
Cell Name
CD9 Stable Expression Cell Line (LN-229)
Species
Human
Gene Name
CD9
Passage ratio
1:2-1:4
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=1.5μg/ml
Culture method
90%DMEM+10%FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamehCD9-1CтMeanβ-h_actinCтMeanΔCтMeanΔΔCтExpression Rate
CD9 Stable Expression Cell Line (LN-229)17.05215.0132.039-3.61312.235
LN-229_Ctrl(Puro)21.24515.5935.6520.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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