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CYP11B2 Stable Expression Cell Line (V79)

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CYP11B2 Stable Expression Cell Line (V79)
Size
1*10^6
Culture method
90%RPMI-1640+10%FBS
Instruction
Detailed Product Information
CYP11B2 Stable Expression Cell Line (V79)

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.

Product category
Stable Cell Lines
Transcript
NM_000457.6
Cell Name
CYP11B2 Stable Expression Cell Line (V79)
Species
Human
Gene Name
CYP11B2
Passage ratio
1:2-1:3
Cell Morphology
Epithelial-like, adheren
Antibiotic Concentration
Puro=4μg/ml
Culture method
90%RPMI-1640+10%FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NameCYP11B2-2CтMeanQ-CHOK1CтMeanΔCтMeanΔΔCтExpression Rate
CYP11B2 Stable Expression Cell Line (V79)24.90819.1685.740-9.349652.123
V79_Ctrl(Puro)35.38420.29515.0890.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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