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GSK3B Stable Expression Cell Line (HEK293)

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GSK3B Stable Expression Cell Line (HEK293)
Size
1*10^6
Culture method
90%DMEM+10% FBS
Instruction
STR Report
Detailed Product Information
GSK3B Stable Expression Cell Line (HEK293)

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

Product category
Stable Cell Lines
Transcript
NM_001146156.2
Cell Name
GSK3B Stable Expression Cell Line (HEK293)
Species
Human
Gene Name
GSK3B
Passage ratio
1:3~1:6
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=0.5μg/ml
Culture method
90%DMEM+10% FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamehGSK3B-1CтMeanβ-h_actinCтMeanΔCтMeanΔΔCтExpression Rate
GSK3B Stable Expression Cell Line (HEK293)22.04115.5726.469-4.83828.601
HEK293_Ctrl(Puro)26.22114.91411.3070.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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