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MAPRE1 Stable Expression Cell Line (293T)

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MAPRE1 Stable Expression Cell Line (293T)
Size
1*10^6
Culture method
90% DMEM+10%FBS
Instruction
STR Report
Detailed Product Information
MAPRE1 Stable Expression Cell Line (293T)

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.

Product category
Stable Cell Lines
Transcript
NM_012325.3
Cell Name
MAPRE1 Stable Expression Cell Line (293T)
Species
Human
Gene Name
MAPRE1
Passage ratio
1:3~1:6
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=1μg/ml
Culture method
90% DMEM+10%FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NameMAPRE1CтMeanh_actin-3CтMeanΔCтMeanΔΔCтExpression Rate
MAPRE1 Stable Expression Cell Line (293T)17.56415.3662.198-1.6543.147
293T_Ctrl(Puro)18.65514.8033.8520.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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