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MEF2A Stable Expression Cell Line (Hep G2)

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MEF2A Stable Expression Cell Line (Hep G2)
Size
1*10^6
Culture method
90% DMEM+10% FBS
Instruction
STR Report
Detailed Product Information
MEF2A Stable Expression Cell Line (Hep G2)

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.

Product category
Stable Cell Lines
Transcript
NM_001319206.4
Cell Name
MEF2A Stable Expression Cell Line (Hep G2)
Species
Human
Gene Name
MEF2A
Passage ratio
1:2~1:4
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=1μg/ml
Culture method
90% DMEM+10% FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample Name hMEF2A-1CтMeanβ-h_actinCтMeanΔCтMeanΔΔCтExpression Rate
MEF2A Stable Expression Cell Line (Hep G2)19.86315.7144.149-3.2769.687
HepG2_Ctrl(Puro)22.77315.3487.4250.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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