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MRPL40 Stable Expression Cell Line (22RV1)

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MRPL40 Stable Expression Cell Line (22RV1)
Size
1*10^6
Culture method
90% RPMI-1640+10% FBS
Instruction
STR Report
Detailed Product Information
MRPL40 Stable Expression Cell Line (22RV1)

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Deletions in this gene may contribute to the etiology of velo-cardio-facial syndrome and DiGeorge syndrome.

Product category
Stable Cell Lines
Transcript
NM_003776.4
Cell Name
MRPL40 Stable Expression Cell Line (22RV1)
Species
Human
Gene Name
MRPL40
Passage ratio
1:2~1:3
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=4μg/ml
Culture method
90% RPMI-1640+10% FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamehMRPL40-1CтMeanβ-h_actinCтMeanΔCтMeanΔΔCтExpression Rate
MRPL40 Stable Expression Cell Line (22RV1)15.98914.9271.062-4.30919.822
22RV1_Ctrl(Puro)19.66114.295.3710.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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