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SCN5A Stable Expression Cell Line (HEK293)

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SCN5A Stable Expression Cell Line (HEK293)
Size
1*10^6
Culture method
90%DMEM+10% FBS
Instruction
STR Report
Detailed Product Information
SCN5A Stable Expression Cell Line (HEK293)

The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in several transcript variants encoding different isoforms.

Product category
Stable Cell Lines
Transcript
NM_198056.3
Cell Name
SCN5A Stable Expression Cell Line (HEK293)
Species
Human
Gene Name
SCN5A
Passage ratio
1:3~1:6
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=0.5μg/ml
Culture method
90%DMEM+10% FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamehSCN5A-1CтMeanβ-h_actinCтMeanΔCтMeanΔΔCтExpression Rate
SCN5A Stable Expression Cell Line (HEK293)16.83614.2082.628-14.41521844.766
HEK293_Ctrl(Puro)31.81314.7717.0430.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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