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TSLP Stable Expression Cell Line (293T)

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TSLP Stable Expression Cell Line (293T)
Size
1*10^6
Culture method
90% DMEM+10%FBS
Instruction
STR Report
Detailed Product Information
TSLP Stable Expression Cell Line (293T)

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants.

Product category
Stable Cell Lines
Transcript
NM_033035.5
Cell Name
TSLP Stable Expression Cell Line (293T)
Species
Human
Gene Name
TSLP
Passage ratio
1:3~1:6
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=1μg/ml
Culture method
90% DMEM+10%FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NamehTSLPCтMeanβ-h_actinCтMeanΔCтMeanΔΔCтExpression Rate
TSLP Stable Expression Cell Line (293T)16.20716.0590.148-11.7863531.340
293T_Ctrl(Puro)27.71915.78511.9340.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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