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USP10 Stable Expression Cell Line (HEK293)

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USP10 Stable Expression Cell Line (HEK293)
Size
1*10^6
Culture method
90%DMEM+10% FBS
Instruction
STR Report
Detailed Product Information
USP10 Stable Expression Cell Line (HEK293)

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene.

Product category
Stable Cell Lines
Transcript
NM_001272075.2
Cell Name
USP10 Stable Expression Cell Line (HEK293)
Species
Human
Gene Name
USP10
Passage ratio
1:3~1:6
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=0.5μg/ml
Culture method
90%DMEM+10% FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NameUSP10-3CтMeanβ-h_actinCтMeanΔCтMeanΔΔCтExpression Rate
USP10 Stable Expression Cell Line (HEK293)20.71618.6162.100-16.13872114.413
HEK293_Ctrl(Puro)3819.76218.2380.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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