A-375-CAS9 cell line

EN CN
Scientist.com
Approved Supplier
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
ENCN
location： Home > Products > Cas9 Stable Cell Lines > A-375-CAS9
KO Cell Lines
Stable Cell Line
Wild-type Cell Lines
Stem Cell Lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
  • A-375 A-375-CAS9-A-375
  • A-375-CAS9 cell line A-375-CAS9-产品图
A-375 A-375-CAS9-A-375
A-375-CAS9 cell line A-375-CAS9-产品图
A-375-CAS9 Copy Product Info
Catalog#： YC-C036-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
STR Report
Datasheet (Use Instruction)
  • A-375 A-375-CAS9-A-375
  • A-375-CAS9 cell line A-375-CAS9-产品图
A-375-CAS9 Copy Product Info
Catalog#： YC-C036-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%DMEM+10%FBS
Passage ratio 1:3~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%DMEM+10%FBS
Passage ratio
1:3~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's A-375 cell line was constructed on the basis of wild-type A-375 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in A-375 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
A-375-CAS9 Cas9 15.59495449 -0.27741051
A-375-CAS9 β-actin 15.87236500
A-375 Cas9 35.02643967 18.13689614
A-375 β-actin 16.88954353
Cutting Efficiency Validation
A-375-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the A-375-CAS9 stable cell pool which is electroporated by CMTM6 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:A-375		 STR Info (Cell bank)
Cell Line:A-375 [A375]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11141114
D7S820 9999
D16S539 9999
VWA 16171617
TH01 8888
AMEL XXXX
TPOX 810810
CSF1PO 11121112
D12S391 1821
FGA 2323
D2S1338 1624
D21S11 2930
D18S51 1217
D8S1179 1114
D3S1358 1517
D6S1043 1114
PENTAE 1012
D19S433 1314.2
PENTAD 915
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>
Learn More>>>

Related Popular Products

KO Cell Lines

  • HLA-A Knockout cell line (A-375)

    HLA-A Knockout cell line (A-375)
      inquiry   More details

    Organism:
    Gene Name: HLA-A
    Gene ID: 3105
    Size: 1*10^6
    Catalog#: YKO-H1439
    YKO-H1439(A-375)
  • PRAME Knockout cell line (A-375)

    PRAME Knockout cell line (A-375)
      inquiry   More details

    Organism: Human
    Gene Name: PRAME
    Gene ID: 23532
    Size: 1*10^6
    Catalog#: YKO-H1073
    YKO-H1073(A-375)
  • B3GNT2 Knockout cell line (A-375)

    B3GNT2 Knockout cell line (A-375)
      inquiry   More details

    Organism: Human
    Gene Name: B3GNT2
    Gene ID: 10678
    Size: 1*10^6
    Catalog#: YKO-H486
    YKO-H486(A-375)

EGFP Stable Cell Lines

Wild-type Cell Lines

Luciferase Stable Cell Lines

Related Popular Products

KO Cell Lines

  • HLA-A Knockout cell line (A-375)

    HLA-A Knockout cell line (A-375)

    Organism:
    Gene Name: HLA-A
    Gene ID: 3105
    Size: 1*10^6
    Catalog#: YKO-H1439
      inquiry   More details
  • PRAME Knockout cell line (A-375)

    PRAME Knockout cell line (A-375)

    Organism: Human
    Gene Name: PRAME
    Gene ID: 23532
    Size: 1*10^6
    Catalog#: YKO-H1073
      inquiry   More details
  • B3GNT2 Knockout cell line (A-375)

    B3GNT2 Knockout cell line (A-375)

    Organism: Human
    Gene Name: B3GNT2
    Gene ID: 10678
    Size: 1*10^6
    Catalog#: YKO-H486
      inquiry   More details

EGFP Stable Cell Lines

Wild-type Cell Lines

Luciferase Stable Cell Lines


WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us
✖

Contact us

If we are unable to reach you via email, how else can we contact you?
✖
Submission Succeeded
Book A Virtual Meeting With Technical Support