A549-CAS9

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A549-CAS9

A549-CAS9 Order

Catalog#YC-C016-Cas9-H

Price (USD)-

Size 1*10^6

Instruction

A549 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Cell Info

Catalog YC-C016-Cas9-H
Cell line A549-CAS9 Cell Type Human Lung Cancer Cell Line
Morphology Epithelial-like, adherent Passage ratio 1:3~1:5
Culture method 90% F12K+10% FBS
Cryopreservation solution 50%F12K+40%FBS+10%DMSO
Special Note

Product Validation Data

RT-QPCR
Sample NameTarget NameCт MeanΔCт
A549-CAS9Cas915.903895380.20190239
A549-CAS9h-actin-B15.70199299
A549Cas935.6263771120.59250927
A549h-actin-B15.03386784
Cutting Efficiency Validation
Note: The above figure shows the sequencing peaks of the A549-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

  • (1)Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • (2)High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • (3)High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Images

A549

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