HL-1-CAS9

Gene Editing Services

Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide.

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EZ-editor™
Gene-editing Series Products

With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines, fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved, achieving our aim of 'Make genome editing easier'!

Red Cotton™ gRNA Plasmid Bank

Regular Plasmid

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Monoclone Genotype Validation Kit (extraction free)

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Point Mutation gDNA/Cell Line

Red Cotton Gene knockout Project

Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases.

Red Cotton CRISPR Gene Editing Designer

Gene Editing Cell Designer

Gene Editing Microbe Designer

Gene Editing Tool Kit

Red Cotton Gene knockout Project

location： Home > Products > Cas9 Stable Cell Lines > HL-1-CAS9

gRNA Plasmid Bank

Cell Lines

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Skeleton, Articulus, Soft Tissue, Derma System

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Cas9 Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

Luciferase Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

EGFP Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

Stem Cell Lines

Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

Lentivirus

All products

HL-1-CAS9 Order

Catalog#：YC-C100-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

HL-1 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-C100-Cas9-H
Cell line	HL-1-CAS9	Cell Type	Mouse cardiac muscle cell line HL-1
Morphology	Fibroblast-like, adherent	Passage ratio	1：2-1：4
Culture method	90%DMEM+10%FBS
Cryopreservation solution	50%DMEM+40%FBS+10%DMSO
Special Note

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
HL-1-CAS9	Cas9	16.68845558	4.75841999
HL-1-CAS9	h-actin-B	11.93003559	4.75841999
HL-1	Cas9	31.03534508	18.67479229
HL-1	h-actin-B	12.36055279	18.67479229

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the HL-1-CAS9 stable cell pool which is electroporated by Kras gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.