ISHIKAWA-CAS9 cell line

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ISHIKAWA-CAS9 Order

ISHIKAWA-CAS9 cell line
Catalog#
YC-C052-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ISHIKAWA-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
ISHIKAWA-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:3-1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
84%MEM+15%FBS+1%NEAA
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the ISHIKAWA cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a ISHIKAWA-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
ISHIKAWA-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the ISHIKAWA-CAS9 stable cell pool which is electroporated by SDHB gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Loci STR Info (Sample Cell)
Sample Cell Line:ISHIKAWA
STR Info (Cell bank)
Cell Line:Ishikawa
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
D5S818 10111011
D13S317 912912
D7S820 910910
D16S539 9999
VWA 14171417
TH01 910910
AMEL XXXX
TPOX 8888
CSF1PO 12131112
D12S391 181920.021.0
FGA 202122.0
D2S1338 1921
D21S11 2828
D18S51 1220
D8S1179 1316
D3S1358 1617
D6S1043 1218
PENTAE 1119
D19S433 12.214
PENTAD 1011
D1S1656 131415.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
ISHIKAWA-CAS9 cell line
ISHIKAWA-CAS9
Catalog#
YC-C052-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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