KG-1a-CAS9 cell line

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KG-1a-CAS9 Order

KG-1a-CAS9 cell line
Catalog#
YC-C083-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KG-1a-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
KG-1a-CAS9
Morphology
Lymphoblast,suspension
Passage ratio
Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the KG-1a cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a KG-1a-CAS9 cell line that can maintain long-term, stable and high expression.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:KG-1a
STR Info (Cell bank)
Cell Line:KG-1a
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 810810
D16S539 10111011
VWA 14191419
TH01 7878
AMEL XYXY
TPOX 7979
CSF1PO 7777
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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