L-929-CAS9

Gene Editing Services

Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide.

Stable Cell Lines

Knockout Cell Lines

Knockin Cell Lines

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iPSC and ESC Gene editing

Cloning Services

Knockout Plasmid

Point Mutation Plasmid

Knockin Plasmid

Overexpression Plasmid

Knockdown Plasmid

Virus Packaging

Lentivirus Packaging

Adenovirus Packaging

Adeno-associated Virus Packaging

CRISPR Library

CRISPR Library In-stock

Customized CRISPR Library

CRISPR Library Screening

Microbe

Gene-editing E.coli

Gene-editing Salmonella

Gene-editing P. aeruginosa

Gene-editing S. aureus

Cell Assay

Cell Proliferation

Cell Migration and Invasion

Cell Cycle

Cell Apoptosis

Detection of Marker Protein Expression

Cytokine Secretion and Enzyme Activity Test

Cytotoxicity Test

EZ-editor™
Gene-editing Series Products

With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines, fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved, achieving our aim of 'Make genome editing easier'!

Red Cotton™ gRNA Plasmid Bank

Regular Plasmid

Lentiviral Plasmid

EZ-editor™ Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ Cell Freezing Medium

Knockout-cell-lysate

KO cell lysates (clone)

KO cell lysates (pool)

EZ-editor™ Cell Line

KO Cell Lines

Wild-type Cell Lines

Cas9 Stable Cell Lines

Luciferase Stable Cell Lines

EGFP Stable Cell Lines

EZ-editor™ Kits

Monoclone Genotype Validation Kit (extraction free)

EZ-editor™ Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

Lentivirus

IVD positive controls

Point Mutation gDNA/Cell Line

Red Cotton Gene knockout Project

Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases.

Red Cotton CRISPR Gene Editing Designer

Gene Editing Cell Designer

Gene Editing Microbe Designer

Gene Editing Tool Kit

Red Cotton Gene knockout Project

location： Home > Products > Cas9 Stable Cell Lines > L-929-CAS9

gRNA Plasmid Bank

Cell Lines

KO Cell Lines

Wild-type Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

Cas9 Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

Luciferase Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

EGFP Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

Stem Cell Lines

Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

Lentivirus

All products

L-929-CAS9 Order

Catalog#：YC-C091-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

L-929 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-C091-Cas9-H
Cell line	L-929-CAS9	Cell Type	Mouse Fibroblast Cell Line
Morphology	Fibroblast，adherent	Passage ratio	1:2~1:5
Culture method	90%MEM+10% HS
Cryopreservation solution	50%MEM+40%HS+10%DMSO
Special Note

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
L-929-CAS9	Cas9	20.18907166	1.02870751
L-929-CAS9	h-actin-B	19.16036415	1.02870751
L-929	Cas9	33.88087463	13.90340232
L-929	h-actin-B	19.97747231	13.90340232

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the L-929-CAS9 stable cell pool which is electroporated by Vav3 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.