LS 174T-CAS9 cell line

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  • LS 174T-CAS9 cell line LS 174T-CAS9-产品图
LS 174T-CAS9 cell line LS 174T-CAS9-产品图
LS 174T-CAS9 Copy Product Info
Catalog#： YC-C088-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10% FBS
STR Report
  • LS 174T-CAS9 cell line LS 174T-CAS9-产品图
LS 174T-CAS9 Copy Product Info
Catalog#： YC-C088-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10% FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like,adherent Culture method 90%DMEM+10% FBS
Passage ratio 1:2~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like,adherent
Culture method
90%DMEM+10% FBS
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's LS 174T cell line was constructed on the basis of wild-type LS 174T cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in LS 174T cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

Cutting Efficiency Validation
LS 174T-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the LS-174T-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:LS 174T		 STR Info (Cell bank)
Cell Line:LS174T
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 1116111415.0
D13S317 10101010
D7S820 11111111
D16S539 11131113
VWA 151617.0151718.0
TH01 6767
AMEL XXXX
TPOX 8989
CSF1PO 10131014
D12S391 1620
FGA 202122.0
D2S1338 1822
D21S11 293031.0
D18S51 1113
D8S1179 1216
D3S1358 1517
D6S1043 121314.0
PENTAE 1516
D19S433 1415
PENTAD 1010
D1S1656 121318.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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