MDA-MB-468-CAS9 cell line

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  • MDA-MB-468-CAS9 cell line MDA-MB-468-CAS9-产品图
MDA-MB-468-CAS9 cell line MDA-MB-468-CAS9-产品图
MDA-MB-468-CAS9 Copy Product Info
Catalog#： YC-D009-Cas9-H
Size： 1*10^6
Culture method： 90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
STR Report
Datasheet (Use Instruction)
  • MDA-MB-468-CAS9 cell line MDA-MB-468-CAS9-产品图
MDA-MB-468-CAS9 Copy Product Info
Catalog#： YC-D009-Cas9-H
Size： 1*10^6
Culture method： 90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
Passage ratio 1:2~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's MDA-MB-468 cell line was constructed on the basis of wild-type MDA-MB-468 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in MDA-MB-468 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
MDA-MB-468-CAS9 Cas9 14.63675308 -0.05153752
MDA-MB-468-CAS9 β-actin 14.68829060
MDA-MB-468 Cas9 37.40849686 21.26742363
MDA-MB-468 β-actin 16.14107323
Cutting Efficiency Validation
MDA-MB-468-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the MDA-MB-468-CAS9 stable cell pool which is electroporated by NLGN4X gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:MDA-MB-468		 STR Info (Cell bank)
Cell Line:MDA-MB-468
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 12121212
D7S820 8888
D16S539 9999
VWA 18181818
TH01 7777
AMEL XXXX
TPOX 8989
CSF1PO 12121212
D12S391 1818
FGA 2323
D2S1338 2525
D21S11 2728
D18S51 1717
D8S1179 1313
D3S1358 1515
D6S1043 1323
PENTAE 55
D19S433 1213
PENTAD 810
D1S1656 1114
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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