MGC803-CAS9 cell line

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MGC803-CAS9 (Hela-contaminated cells, not for sales) Order

MGC803-CAS9 cell line
Catalog#
YC-C081-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MGC803-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
MGC803-CAS9
Morphology
Epithelial-like,adherent
Passage ratio
1:3-1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the MGC803 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a MGC803-CAS9 cell line that can maintain long-term, stable and high expression.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:MGC803
STR Info (Cell bank)
Cell Line:MGC-803
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
D5S818 101112.0101112.0
D13S317 713.3713.3
D7S820 11121112
D16S539 911911
VWA 161718.0161718.0
TH01 7979
AMEL XXXX
TPOX 12121212
CSF1PO 91012.091012.0
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 191110). MGC-803 was reported to originally come from gastric carcinoma but several papers from cell repositories in China have shown that it has a high similarity to HeLa. Looking at the STR profiles that we have available, they appear to fall into two groups. One is consistent with HeLa, while the other has additional alleles at many loci e.g. Penta E has "7,10,16,17". This second group of samples may represent a mixture of HeLa and a second cell line; it may also represent a hybrid cell line (e.g., following exposure to a fusogenic agent).

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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