NCI-H1299-CAS9 cell line

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > Cas9 Stable Cell Lines > NCI-H1299-CAS9

     gRNA Plasmid Bank

     Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

     Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

     Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

     Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

NCI-H1299-CAS9 Order

NCI-H1299-CAS9 cell line
Catalog#
YC-D010-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium(NCI-H1299)
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
NCI-H1299-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
NCI-H1299-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the NCI-H1299 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NCI-H1299-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
NCI-H1299-CAS9 Cas9 16.43360901 -0.74985313
NCI-H1299-CAS9 h-actin-B 17.18346214
NCI-H1299 Cas9 36.54208755 20.83538532
NCI-H1299 h-actin-B 15.70670223
Cutting Efficiency Validation
NCI-H1299-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the NCI-H1299-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1299		 STR Info (Cell bank)
Cell Line:NCI-H1299-Luc2-tdT-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 12121212
D7S820 10101010
D16S539 12131213
VWA 16181618
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

Related Popular Products

KO Cell Lines

  • SGMS2 Knockout cell line (NCI-H1299)

    SGMS2 Knockout cell line (NCI-H1299)
      inquiry   More details

    Organism: Human
    Gene Name: SGMS2
    Gene ID: 166929
    Size: 1*10^6
    Catalog#: YKO-H865
    YKO-H865(NCI-H1299)
  • ZDHHC5 Knockout cell line (NCI-H1299)

    ZDHHC5 Knockout cell line (NCI-H1299)
      inquiry   More details

    Organism: Human
    Gene Name: ZDHHC5
    Gene ID: 25921
    Size: 1*10^6
    Catalog#: YKO-H795
    YKO-H795(NCI-H1299)
  • CCDC86 Knockout cell line(NCI-H1299)

    CCDC86 Knockout cell line(NCI-H1299)
      inquiry   More details

    Organism: Human
    Gene Name: CCDC86
    Gene ID: 79080
    Size: 1*10^6
    Catalog#: YKO-H516
    YKO-H516(NCI-H1299)

Related Popular Products

KO Cell Lines

  • SGMS2 Knockout cell line (NCI-H1299)

    SGMS2 Knockout cell line (NCI-H1299)

    Organism: Human
    Gene Name: SGMS2
    Gene ID: 166929
    Size: 1*10^6
    Catalog#: YKO-H865
      inquiry   More details
  • ZDHHC5 Knockout cell line (NCI-H1299)

    ZDHHC5 Knockout cell line (NCI-H1299)

    Organism: Human
    Gene Name: ZDHHC5
    Gene ID: 25921
    Size: 1*10^6
    Catalog#: YKO-H795
      inquiry   More details
  • CCDC86 Knockout cell line(NCI-H1299)

    CCDC86 Knockout cell line(NCI-H1299)

    Organism: Human
    Gene Name: CCDC86
    Gene ID: 79080
    Size: 1*10^6
    Catalog#: YKO-H516
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us