NIH/3T3-CAS9 cell line

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  • NIH/3T3-CAS9 cell line NIH/3T3-CAS9-产品图
NIH/3T3-CAS9 cell line NIH/3T3-CAS9-产品图
NIH/3T3-CAS9 Copy Product Info
Catalog#： YC-A013-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
STR Report
Datasheet (Use Instruction)
  • NIH/3T3-CAS9 cell line NIH/3T3-CAS9-产品图
NIH/3T3-CAS9 Copy Product Info
Catalog#： YC-A013-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Fibroblast-like, adherent Culture method 90%DMEM+10%FBS
Passage ratio 1:3~1:5 Species Mouse
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Fibroblast-like, adherent
Culture method
90%DMEM+10%FBS
Passage ratio
1:3~1:5
Species
Mouse
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's NIH/3T3 cell line was constructed on the basis of wild-type NIH/3T3 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in NIH/3T3 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
NIH/3T3-CAS9 Cas9 16.91583252 3.60078049
NIH/3T3-CAS9 β-actin 13.31505203
NIH/3T3 Cas9 33.85106277 20.10673046
NIH/3T3 β-actin 13.74433231
Cutting Efficiency Validation
NIH/3T3-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the NIH/3T3-CAS9 stable cell pool which is electroporated by Tmem65 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:NIH/3T3		 STR Info (Cell bank)
Cell Line:NIH 3T3
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.019.017.019.0
4-2 19.320.319.320.3
6-7 12.012.0
19-2 10.011.012.011.012.0
1-2 13.017.013.017.0
7-1 29.029.0
8-1 15.015.0
1-1 10.010.0
3-2 14.015.014.015.0
2-1 9.09.0
15-3 20.320.3
6-4 16.015.3
13-1 16.216.2
11-2 15.017.015.017.0
17-2 13.014.013.014.0
12-1 20.020.0
5-5 14.015.014.015.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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