NIH/3T3-CAS9

Gene Editing Services

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With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines, fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved, achieving our aim of 'Make genome editing easier'!

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location： Home > Products > Cas9 Stable Cell Lines > NIH/3T3-CAS9

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Cas9 Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

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Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

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Luciferase Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

EGFP Stable Cell Lines

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Brain and Nervous System

Circulatory System

Reproductive System

Urinary System

Blood and Lymphatic System

Respiratory System

Endocrine System

Digestive System

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Transfection Culture Medium

Cell Monoclonal Culture Medium

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NIH/3T3-CAS9 Order

Catalog#：YC-A013-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

NIH/3T3 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-A013-Cas9-H
Cell line	NIH/3T3-CAS9	Cell Type	Mouse Embryonic Fibroblasts
Morphology	Fibroblast-like, adherent	Passage ratio	1:3~1:5
Culture method	90%DMEM+10%FBS
Cryopreservation solution	50%DMEM+40%FBS+10%DMSO
Special Note

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
NIH/3T3-CAS9	Cas9	16.91583252	3.60078049
NIH/3T3-CAS9	h-actin-B	13.31505203	3.60078049
NIH/3T3	Cas9	33.85106277	20.10673046
NIH/3T3	h-actin-B	13.74433231	20.10673046

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the NIH/3T3-CAS9 stable cell pool which is electroporated by Kras gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.