SGC-7901-CAS9 cell line

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SGC-7901-CAS9 (Hela-contaminated cells, not for sales) Order

SGC-7901-CAS9 cell line
Catalog#
YC-C055-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SGC-7901-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
SGC-7901-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:3-1:5
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the SGC-7901 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a SGC-7901-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
SGC-7901-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the SGC-7901-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SGC-7901
STR Info (Cell bank)
Cell Line:SGC-7901
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
D12S391 2025
FGA 2121
D2S1338 1717
D21S11 2728
D18S51 1616
D8S1179 1213
D3S1358 1518
D6S1043 1819
PENTAE 717
D19S433 1314
PENTAD 815
D1S1656 1215
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 190616). SGC-7901 was reported to come from gastric adenocarcinoma but multiple STR profiles correspond to HeLa.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
SGC-7901-CAS9 cell line
SGC-7901-CAS9
Catalog#
YC-C055-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Instruction: Human Gastric Cancer Cell Line(SGC-7901)

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