SK-BR-3-CAS9 cell line

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location: Home > Products > Cas9 Stable Cell Lines > SK-BR-3-CAS9
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  • SK-BR-3 SK-BR-3-CAS9-SK-BR-3
  • SK-BR-3-CAS9 cell line SK-BR-3-CAS9-产品图
SK-BR-3-CAS9 Copy Product Info
Catalog#: YC-C048-Cas9-H
Size: 1*10^6
Culture method: 90%McCOY's 5A+10%FBS
  • SK-BR-3 SK-BR-3-CAS9-SK-BR-3
  • SK-BR-3-CAS9 cell line SK-BR-3-CAS9-产品图
SK-BR-3-CAS9 Copy Product Info
Catalog#: YC-C048-Cas9-H
Size: 1*10^6
Culture method: 90%McCOY's 5A+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%McCOY's 5A+10%FBS
Passage ratio 1:2~1:3 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%McCOY's 5A+10%FBS
Passage ratio
1:2~1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's SK-BR-3 cell line was constructed on the basis of wild-type SK-BR-3 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in SK-BR-3 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
SK-BR-3-CAS9 Cas9 14.79796696 2.00070763
SK-BR-3-CAS9 β-actin 12.79725933
SK-BR-3 Cas9 33.42461395 20.89612579
SK-BR-3 β-actin 12.52848816
Cutting Efficiency Validation
SK-BR-3-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the SK-BR-3-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SK-BR-3
STR Info (Cell bank)
Cell Line:SK-BR-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 912912
D13S317 11121112
D7S820 99912
D16S539 9999
VWA 17171717
TH01 8989
AMEL XXXX
TPOX 811811
CSF1PO 12121212
D12S391 1820
FGA 2020
D2S1338 2025
D21S11 3030.2
D18S51 1013
D8S1179 1212
D3S1358 1717
D6S1043 1119
PENTAE 1011
D19S433 1414
PENTAD 912
D1S1656 1117.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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