SU-DHL-4-CAS9

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SU-DHL-4-CAS9

SU-DHL-4-CAS9 Order

Catalog#YC-C082-Cas9-H

Price (USD)-

Size 1*10^6

Instruction

SU-DHL-4 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Cell Info

Catalog YC-C082-Cas9-H
Cell line SU-DHL-4-CAS9 Cell Type Human Skin Fibroblast Cell Line
Morphology Lymphoblast, suspension Passage ratio Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Culture method 90%RPMI-1640+10% FBS
Cryopreservation solution 50%RPMI-1640+40% FBS+10%DMSO
Special Note The cell culture density needs to be strictly controlled. The cell viability after thawing is relatively low, it is recommended to use Australia-sourced FBS for thawing or increase the FBS ratio to 20%. When the cells grow normally and can be passaged after 2 passages, the cells can be cultured using the normal culture method.

Product Advantage

  • (1)Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • (2)High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • (3)High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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