SW 1353-CAS9 cell line

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SW 1353-CAS9 cell line

SW 1353-CAS9 cell line SW 1353-CAS9-产品图

SW 1353-CAS9 Copy Product Info

Catalog#： YC-C105-Cas9-H

Size： 1*10^6

Culture method： 90%L15+10%FBS

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STR Report

SW 1353-CAS9 cell line

SW 1353-CAS9 Copy Product Info

Catalog#： YC-C105-Cas9-H

Size： 1*10^6

Culture method： 90%L15+10%FBS

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STR Report

Detailed Product Information

Cell Info

Product category	Cas9 Stable Cell Lines	Mycoplasma test	negative
Cell Morphology	Epithelial-like, adherent	Culture method	90%L15+10%FBS
Passage ratio	1:2-1:3	Species	Human
Expression gene	Cas9	Resistance gene	hygro

Product category

Cas9 Stable Cell Lines

Mycoplasma test

negative

Cell Morphology

Epithelial-like, adherent

Culture method

90%L15+10%FBS

Passage ratio

1:2-1:3

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's SW 1353 cell line was constructed on the basis of wild-type SW 1353 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in SW 1353 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

STR Profile

Download STR Report

Loci	STR Info (Sample Cell) Sample Cell Line:SW 1353			STR Info (Cell bank) Cell Line:SW 1353 [SW 1353, SW-1353]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	11		10	11
D13S317	12	13		12	13
D7S820	9	11		9	11
D16S539	11	12		11	12
VWA	16	17		16	17
TH01	6	9		6	9
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	12	12		12	12
D12S391	22	23
FGA	22	23
D2S1338	19	24
D21S11	30	32.2
D18S51	13	17
D8S1179	10	11
D3S1358	15	18
D6S1043	12	14
PENTAE	12	14
D19S433	14	14
PENTAD	10	11
D1S1656	11	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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SW 1353-CAS9 cell line

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