THP-1-CAS9 cell line

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location: Home > Products > Cas9 Stable Cell Lines > THP-1-CAS9
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  • THP-1 THP-1-CAS9-THP-1
  • THP-1-CAS9 cell line THP-1-CAS9-产品图
THP-1-CAS9 Copy Product Info
Catalog#: YC-D011-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10%FBS
  • THP-1 THP-1-CAS9-THP-1
  • THP-1-CAS9 cell line THP-1-CAS9-产品图
THP-1-CAS9 Copy Product Info
Catalog#: YC-D011-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Monocyte,suspension Culture method 90%RPMI-1640+10%FBS
Passage ratio 1:2-1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Monocyte,suspension
Culture method
90%RPMI-1640+10%FBS
Passage ratio
1:2-1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's THP-1 cell line was constructed on the basis of wild-type THP-1 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in THP-1 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
THP-1-CAS9 Cas9 18.14743614 4.86366844
THP-1-CAS9 β-actin 13.28376770
THP-1 Cas9 34.46055222 21.80498600
THP-1 β-actin 12.65556622
Cutting Efficiency Validation
THP-1-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the THP-1-CAS9 stable cell pool which is electroporated by UBA7 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:THP-1
STR Info (Cell bank)
Cell Line:THP-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 13131313
D7S820 10101010
D16S539 11121112
VWA 16161616
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 11131113
D12S391 1919
FGA 2425
D2S1338 1718
D21S11 3031.2
D18S51 1314
D8S1179 1014
D3S1358 1517
D6S1043 1414
PENTAE 1115
D19S433 12.213
PENTAD 1012
D1S1656 1617
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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