HBL-100-CAS9 cell line

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HBL-100-CAS9 cell line

HBL-100-CAS9 cell line HBL-100-CAS9-产品图

HBL-100-CAS9 Copy Product Info

* Misidentified source

Catalog#： YC-C013-Cas9-H

Size： 1*10^6

Culture method： 90%RPMI-1640+10%FBS

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HBL-100-CAS9 cell line

HBL-100-CAS9 Copy Product Info

* Misidentified source

Catalog#： YC-C013-Cas9-H

Size： 1*10^6

Culture method： 90%RPMI-1640+10%FBS

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STR Report

Detailed Product Information

Cell Info

Product category	Cas9 Stable Cell Lines	Mycoplasma test	negative
Cell Morphology	Epithelial-like, adherent	Culture method	90%RPMI-1640+10%FBS
Passage ratio	1:2~1:4	Species	Human
Expression gene	Cas9	Resistance gene	hygro

Product category

Cas9 Stable Cell Lines

Mycoplasma test

negative

Cell Morphology

Epithelial-like, adherent

Culture method

90%RPMI-1640+10%FBS

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's HBL-100 cell line was constructed on the basis of wild-type HBL-100 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in HBL-100 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the HBL-100-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HBL-100			STR Info (Cell bank) Cell Line:HBL-100
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	12	12		12	12
D7S820	8	12		8	12
D16S539	9	12		9	12
VWA	16	16		16	16
TH01	6	8		6	8
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	10	10		10	10
D12S391	18	18
FGA	25	25
D2S1338	18	24
D21S11	28	29	30.0
D18S51	16	16
D8S1179	12	15
D3S1358	14	16
D6S1043	12	14
PENTAE	7	7
D19S433	15	15
PENTAD	12	12
D1S1656	12	16.3

* Misidentified source

Product Advantage

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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