HBL-100-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > HBL-100-CAS9
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  • HBL-100-CAS9 cell line HBL-100-CAS9-产品图
HBL-100-CAS9 cell line HBL-100-CAS9-产品图
HBL-100-CAS9 Copy Product Info
* Misidentified source
Catalog#： YC-C013-Cas9-H
Size： 1*10^6
Culture method： 90%RPMI-1640+10%FBS
  • HBL-100-CAS9 cell line HBL-100-CAS9-产品图
HBL-100-CAS9 Copy Product Info
* Misidentified source
Catalog#： YC-C013-Cas9-H
Size： 1*10^6
Culture method： 90%RPMI-1640+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%RPMI-1640+10%FBS
Passage ratio 1:2~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%RPMI-1640+10%FBS
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's HBL-100 cell line was constructed on the basis of wild-type HBL-100 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in HBL-100 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

Cutting Efficiency Validation
HBL-100-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HBL-100-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HBL-100		 STR Info (Cell bank)
Cell Line:HBL-100
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 12121212
D7S820 812812
D16S539 912912
VWA 16161616
TH01 6868
AMEL XXXX
TPOX 8888
CSF1PO 10101010
D12S391 1818
FGA 2525
D2S1338 1824
D21S11 282930.0
D18S51 1616
D8S1179 1215
D3S1358 1416
D6S1043 1214
PENTAE 77
D19S433 1515
PENTAD 1212
D1S1656 1216.3
* Misidentified source

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>
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