IPSC-DYR0100-CAS9 cell line

EN CN
Scientist.com
Approved Supplier
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
ENCN
location： Home > Products > Cas9 Stable Cell Lines > IPSC-DYR0100-CAS9
KO Cell Lines
Stable Cell Lines
Wild-type Cell Lines
Stem Cell Lines
All products
  • All products
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • OVA Stable Cell Lines
  • Stable Cell Lines
  • IPSC-DYR0100 IPSC-DYR0100-CAS9-IPSC-DYR0100
  • IPSC-DYR0100-CAS9 cell line IPSC-DYR0100-CAS9-产品图
IPSC-DYR0100 IPSC-DYR0100-CAS9-IPSC-DYR0100
IPSC-DYR0100-CAS9 cell line IPSC-DYR0100-CAS9-产品图
IPSC-DYR0100-CAS9 Copy Product Info
Catalog#： YC-C098-Cas9-H
Size： 1*10^6
Culture method：
Datasheet (Use Instruction)
  • IPSC-DYR0100 IPSC-DYR0100-CAS9-IPSC-DYR0100
  • IPSC-DYR0100-CAS9 cell line IPSC-DYR0100-CAS9-产品图
IPSC-DYR0100-CAS9 Copy Product Info
Catalog#： YC-C098-Cas9-H
Size： 1*10^6
Culture method：
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology -- Culture method --
Passage ratio -- Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
--
Culture method
--
Passage ratio
--
Species
Human
Expression gene
Cas9
Resistance gene
hygro
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's IPSC-DYR0100 cell line was constructed on the basis of wild-type IPSC-DYR0100 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in IPSC-DYR0100 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
IPSC-DYR0100-CAS9 Cas9 21.07497406 7.93462086
IPSC-DYR0100-CAS9 β-actin 13.14035320
IPSC-DYR0100 Cas9 34.49109268 21.41424656
IPSC-DYR0100 β-actin 13.07684612
Cutting Efficiency Validation
IPSC-DYR0100-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the IPSC-DYR0100-CAS9 stable cell pool which is electroporated by ABCA gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>
Learn More>>>

Related Popular Products

Stable Cell Lines

KO Cell Lines

  • ARHGEF18 Knockout cell line (IPSC)

    ARHGEF18 Knockout cell line (IPSC)
    Request a Quote More details

    Organism: Human
    Gene Name: ARHGEF18
    Gene ID: 23370
    Size: 1*10^6
    Catalog#: YKO-H985
    YKO-H985(IPSC-DYR0100)
  • SVEP1 Knockout cell line (Vips)

    SVEP1 Knockout cell line (Vips)
    Request a Quote More details

    Organism: Human
    Gene Name: SVEP1
    Gene ID: 79987
    Size: 1*10^6
    Catalog#: YKO-H984
    YKO-H984(IPSC-DYR0100)
  • SLC25A47 Knockout cell line (Vips)

    SLC25A47 Knockout cell line (Vips)
    Request a Quote More details

    Organism: Human
    Gene Name: SLC25A47
    Gene ID: 283600
    Size: 1*10^6
    Catalog#: YKO-H870
    YKO-H870(IPSC-DYR0100)

EGFP Stable Cell Lines

Stem Cell Lines

Related Popular Products

Stable Cell Lines

KO Cell Lines

  • ARHGEF18 Knockout cell line (IPSC)

    ARHGEF18 Knockout cell line (IPSC)

    Organism: Human
    Gene Name: ARHGEF18
    Gene ID: 23370
    Size: 1*10^6
    Catalog#: YKO-H985
    Request a Quote More details
  • SVEP1 Knockout cell line (Vips)

    SVEP1 Knockout cell line (Vips)

    Organism: Human
    Gene Name: SVEP1
    Gene ID: 79987
    Size: 1*10^6
    Catalog#: YKO-H984
    Request a Quote More details
  • SLC25A47 Knockout cell line (Vips)

    SLC25A47 Knockout cell line (Vips)

    Organism: Human
    Gene Name: SLC25A47
    Gene ID: 283600
    Size: 1*10^6
    Catalog#: YKO-H870
    Request a Quote More details

EGFP Stable Cell Lines

Stem Cell Lines

  • IPSC-DYR0100 cell line

    Human induced pluripotent stem cells (iPSC)(IPSC-DYR0100)

    Price (USD): -
    Catalog#: YC-C098
    Size: 1*10^6
    Instruction: Human induced pluripotent stem cells (iPSC)(IPSC-DYR0100)
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

ISO
Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now

Online
Inquire

Online Inquire

✖

Contact us

  If email is not available, how else can we reach you?
Search Engine
Email
Recommendations from others
Others
✖

Contact us

If email is not available, how else can we reach you?
Search Engine
Email
Recommendations from others
Others
✖
Submission Succeeded
Book A Virtual Meeting With Technical Support