RAW 264.7-CAS9 cell line

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  • RAW 264.7 RAW 264.7-CAS9-RAW 264.7
  • RAW 264.7-CAS9 cell line RAW 264.7-CAS9-产品图
RAW 264.7 RAW 264.7-CAS9-RAW 264.7
RAW 264.7-CAS9 cell line RAW 264.7-CAS9-产品图
RAW 264.7-CAS9 Copy Product Info
Catalog#： YC-C020-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
STR Report
Datasheet (Use Instruction)
  • RAW 264.7 RAW 264.7-CAS9-RAW 264.7
  • RAW 264.7-CAS9 cell line RAW 264.7-CAS9-产品图
RAW 264.7-CAS9 Copy Product Info
Catalog#： YC-C020-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology monocyte/macrophage,adherent Culture method 90%DMEM+10%FBS
Passage ratio 1:2-1:3 Species Mouse
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
monocyte/macrophage,adherent
Culture method
90%DMEM+10%FBS
Passage ratio
1:2-1:3
Species
Mouse
Expression gene
Cas9
Resistance gene
hygro
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's RAW 264.7 cell line was constructed on the basis of wild-type RAW 264.7 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in RAW 264.7 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
RAW 264.7-CAS9 Cas9 20.70931435 8.58496571
RAW 264.7-CAS9 β-actin 12.12434864
RAW 264.7 Cas9 34.13895035 21.32848454
RAW 264.7 β-actin 12.81046581
Cutting Efficiency Validation
RAW 264.7-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the RAW 264.7-CAS9 stable cell pool which is electroporated by Ppt1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:RAW 264.7		 STR Info (Cell bank)
Cell Line:RAW 264.7
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.018.0
4-2 22.322.3
6-7 12.012.0
19-2
1-2 17.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.0
3-2 14.014.0
2-1 16.016.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 17.017.0
17-2 14.016.014.016.0
12-1 16.016.0
5-5 14.014.0
X-1 24.024.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Citations:
Product Catalog#：YC-C020-Cas9-H
Product Name：RAW 264.7-CAS9
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>
Learn More>>>

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