ACLY Knockout cell line (PANC-1)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > ACLY Knockout cell line (PANC-1)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

ACLY Knockout cell line (PANC-1) Order

ACLY Knockout cell line (PANC-1)
Catalog#
YKO-H555
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ACLY Knockout cell line (PANC-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ACLY Knockout cell line (PANC-1)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
ACLY
Gene ID
47
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 3 (based on transcript ACLY-201)
Validation: PCR+Sanger Sequencing
ACLY Knockout cell line (PANC-1) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:PANC-1
STR Info (Cell bank)
Cell Line:PANC-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 11111111
D7S820 810810
D16S539 11111111
VWA 15151515
TH01 7878
AMEL XXXX
TPOX 811811
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ACLY gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PANC-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ACLY gene knockout ACLY Knockout cell line (PANC-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ACLY Knockout cell line (HeLa)

    ACLY Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ACLY
    Gene ID: 47
    Size: 1*10^6
    Catalog#: YKO-XN85067
    YKO-XN85067(HeLa)
  • ACLY Knockout cell line (HCT 116)

    ACLY Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ACLY
    Gene ID: 47
    Size: 1*10^6
    Catalog#: YKO-XN85066
    YKO-XN85066(HCT 116)
  • ACLY Knockout cell line (HEK293)

    ACLY Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ACLY
    Gene ID: 47
    Size: 1*10^6
    Catalog#: YKO-XN85065
    YKO-XN85065(HEK293)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • ACLY Knockout cell line (HeLa)

    ACLY Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ACLY
    Gene ID: 47
    Size: 1*10^6
    Catalog#: YKO-XN85067
  • ACLY Knockout cell line (HCT 116)

    ACLY Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ACLY
    Gene ID: 47
    Size: 1*10^6
    Catalog#: YKO-XN85066
  • ACLY Knockout cell line (HEK293)

    ACLY Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ACLY
    Gene ID: 47
    Size: 1*10^6
    Catalog#: YKO-XN85065

Contact us