ANKH Knockout cell line (293T)

location： Home > Products > KO Cell Lines > ANKH Knockout cell line (293T)

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

ANKH Knockout cell line (293T) Order

Catalog#：YKO-H846

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H846
Cell line	ANKH Knockout cell line (293T)	Cell Type	Human Embryonic Kidney Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:3~1:6
Culture method	90% DMEM+10%FBS
Cryopreservation solution	50%DMEM+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol

ANKH

Gene id

56172

Official full symbol

ANKH inorganic pyrophosphate transport regulator

Also known as

ANK, CCAL2, CMDJ, CPPDD, HANK, MANK, SLC62A1

This gene encodes a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals. Mutations in this gene have been associated with autosomal dominant craniometaphyseal dysplasia.